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Ruffino d'Assisi also known as Ruffino Bartolucci , Ruffino Bartolucci of Assisi or Fra Ruffin , born around 1490 and died in or after 1532, was a Franciscan monk and Italian Renaissance composer.

He was born about 1490, perhaps in Assisi , whence his name. Brother of the Order of the Franciscans , he is documented in Padua , where he is a chapel master at the cathedral between 1510 and 1520. He was then employed in the Basilica of St. Anthony of Padua until 1525.

Attached to the Cathedral of Vicenza from 1525, he then returned to the Basilica of Saint Anthony, where he is documented until 1532.

His date of death is not known with certainty: 1532 or later.

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